0:00:00in this video we demonstrate a novel method of encapsulating protein drinks in problem eric
0:00:05micro needles
0:00:07the fabrication of micro needles is divided into two stages the fabrication of micro little
0:00:13backing layer followed by fabrication of macaroni do shots
0:00:18at first we will describe the application of micro you go back and we are
0:00:23capri problem resolution contain protein dried bovine serum opium and its first weight into the
0:00:29cavity formed by supporting cover slips and a glass like substrate
0:00:34this setup is then placed on a flat stage below high intensity you v land
0:00:38at a distance of nine point five centimetres from the u v light source
0:00:43it is then exposed to high power you v light for two seconds to polarise
0:00:47the liquid and form the micro needle lacking clear
0:00:51the glass fast rate is then removed from the set up and the backing layer
0:00:55containing cover slips removed from the substrate with the help of a delayed
0:01:00this is followed by fabrication of the macaroni do shots
0:01:04the pre problem resolution is similar to fill in have any form to between the
0:01:08covers like hurting backing layer and the class that straight
0:01:12the length of the micro needle can be controlled by the number of cover slip
0:01:15spacers placed on the base class light
0:01:19one major difference between the fabrication of america muto backing layer and shots is to
0:01:23use of spatially patterns build a mask in this that the voters consists of a
0:01:28possible which is obtained in this header the entrance variability between produced you like to
0:01:34pass through
0:01:35this film is placed on the cover slip are which backing where is attached and
0:01:39the setup is placed on a platform at a distance of three point five centimetres
0:01:44from the u v lights lars
0:01:46it is then exposed to high intensity you v like for three point five seconds
0:01:50to form the micro needles
0:01:52the setup is removed from the stage and the bottle mask is removed with the
0:01:56help of tweezers
0:01:59the latter over pulverised polymer solution is washed under running water and micro needles removed
0:02:05from the substrate and let air dry
0:02:08the fabricated micron heroes were tested for stability of encapsulate protein drugs really using the
0:02:14protein in water and testing is what primary secondary tertiary structure characteristics i asked us
0:02:20page circular victories them and floors and spectroscopy respectively
0:02:26the result shows that structural characteristics of the coding or some larger freshly prepare solutions
0:02:31and significantly different from bs a degraded at high temperature and on their prosody conditions
0:02:36english or weenies demonstrated that be a stay was almost completely released at the end
0:02:41of six hours
0:02:43in visual presentation experience using that scheme should significant enhancement micro needle media coding permissions
0:02:49as compared to pass the diffusion
0:02:52following this by a compatibility of michael needles was assessed by my to control the
0:02:57hijacking this and the likelihood righteousness activity using m t and l t h s
0:03:02is respectively
0:03:05hi cell viability is were observed indicating the bible compatible nature of all the merrick
0:03:10my chronicles