0:00:05my name is fernando sentences i am a senior at the university of arizona majoring
0:00:10and physiology psychology and in spanish translation interpretation but i did my research or the
0:00:16department of physiology of the college of madison
0:00:19for a doctor hendrickson along with would you and process on a wrong and maybe
0:00:24k time and static my research had you with a
0:00:30zurich protein as the steering chinese it's called c g and terminal kind he's or
0:00:35junk for short
0:00:36and it had to do with its contribution to insulin resistance of them aliens skeletal
0:00:43muscle
0:00:43so diabetes is the main overall focus of my work and it's like you disease
0:00:49that's characterized by comically elevated
0:00:52fasting glucose levels in the blood insulin resistance of a million skeletal muscle is the
0:00:57main defect that ultimately leads to the development of type to die one of the
0:01:01ways that we know "'cause" insulin resistance is a condition cox date of stress
0:01:07now octave stresses normally our body creates these things called reactive oxygen species the cell
0:01:13normally has enough mechanisms to prevent them from getting too high
0:01:16when they do get too high this is a condition known as ox data stress
0:01:20and this figure right here shows the relationship between update of stress in the cell
0:01:25the stressed activated steering traces and ultimately insulin resistance we look at as i mentioned
0:01:32the p thirty eight map chinese and the gas key three
0:01:36well but we haven't looked of junk yep of or what we study this is
0:01:40we took healthy lena rats
0:01:44and we cut out there's only a small so that the most on the back
0:01:46to like we incubator the muscle in vitro and we did incubation that to four
0:01:51and six hours this first crap right here i
0:01:54or the glucose uptake results with no insulin hydrogen peroxide there was a significant increase
0:01:59in lucas uptake but the key thing to look at is that with insulin
0:02:04we have as large increase but then we have a significant drop off in we
0:02:08"'cause" uptake in the muscle cell in the presence of the hydrogen peroxide and this
0:02:13was characteristic along all the time points the second drop rate here is look at
0:02:18the interstellar protein expression specific that is one protein call protein training to be or
0:02:23at for short
0:02:25and we looked at this one specifically because we know it's the it's only active
0:02:29when insulin is around these two graph showed us that the issue i don't of
0:02:33occidental supposed to do it decrease glucose uptake it decrease the expression of the active
0:02:38form of a katie essentially indicating that some insulin resistance is occurring in the sound
0:02:43well we're looking at junk specifically which is what this paragraph looked at right here
0:02:47and what the c is junk expressed more the active form the fast correlated form
0:02:53in the presence of hydrogen peroxide and what we found is yes that whether insulin
0:02:58was present or not with the present divided in proc so they were significant increases
0:03:01an active form of john all this tells us right here is that junk is
0:03:05more active in the presence of a state of stress
0:03:08to find of specifically what role chunk has in insulin resistance caused by oxidation stress
0:03:13we had to do one separate experiment which is what this graph over here shows
0:03:19us
0:03:19this is a good "'cause" uptake graphical was different about this is that we incubator
0:03:23the in the presence of a selective junk inhibitor the important for look at it
0:03:27is red ball right here this red ball right here is a theoretical value
0:03:31which we found by combining effects of each individual components this theoretical value is what
0:03:38we would expect to see assuming that junk had nothing to do with insulin resistance
0:03:42that we were seeing here
0:03:43but in fact what we got the experiment was this part right here so this
0:03:47whole difference right here is the fact that junk is having on the glucose uptake
0:03:54within the muscles style and this is a significant increase in what we were expecting
0:03:59to see
0:04:00so what we can conclude is that the activation of this protein john which is
0:04:05a searing chinese has some at least some contribution to the insulin resistance that we
0:04:10see
0:04:11in the million skeletal muscle cost up to date of stress and this suggests that
0:04:15maybe interventions that target jong specifically could alleviate some of this insulin resistance that we
0:04:21see caused by a state of stress