0:00:06she is sabrina device i did research last summer at the university of washington with
0:00:12each region my investigator duct "'em" you're going in my mentor was beyond
0:00:16three and a bottom are
0:00:18and in that lab we were interested in the function of one
0:00:23put one is ubiquitous the express their of than the human body its expressed within
0:00:29a red blood cells individual tissue as well as in the
0:00:32what range very are also important is the fact that we don't quite understand how
0:00:37time mechanism works we hypothesize that the and might be an ideal that the environment
0:00:43for good one to function so we created a system using a one the yeast
0:00:48cell cycle makes easier pca
0:00:50which is the cell because they are you carriers like us and they should have
0:00:54the proper machinery twofold
0:00:57one
0:00:59and we also use the week goes analog called to and beattie g and we
0:01:03are using the speakers
0:01:05us accuracy pca is known to uptake
0:01:09tool beattie g and two in but for s as we can measure glucose uptake
0:01:13we to go past minutes that already had the insert of our could gene which
0:01:20was designed by a previous in turn last
0:01:25summer two thousand ten i mean take that has made with are one gene and
0:01:30transform it into this accuracy seriously results
0:01:34i three
0:01:36that when are you cells uptake our as mids be well correctly falls
0:01:44and
0:01:45hopefully be
0:01:46be good model organism to study
0:01:51to answer this question the role that live it's flea
0:01:54inc one function so there were two constructs of pass minutes that we transformed into
0:01:59our soccer stbc cells
0:02:01one construct had a fist add
0:02:03and of the constructed not have a stack the his study is there so we
0:02:08can easily verify the protein it's we get a visual that the east
0:02:14did express that
0:02:16and the one without the first ad where how it is formed in nature
0:02:22from these pictures it tell those that's accuracy vc is indeed a good model organism
0:02:28to answer this question
0:02:30so not that we have this study completed
0:02:33we can now move on to the next which would be to and to do
0:02:38the actual actually
0:02:40so this task you would involve for two controls
0:02:44before using
0:02:46my transit unique east with the human one insert
0:02:49the first control that we were gonna use is you can buy that is over
0:02:54expressing collectors crewmates
0:02:56and we are gonna used as a control because it
0:03:00equal i
0:03:01has that collections period that is known to of tape to and beattie g so
0:03:06we do are passe it will be confirmation that it is working
0:03:10and next we will have or a while type used to how it's not the
0:03:14nature
0:03:15so we can measure its androgynous
0:03:17that is it's natural ability to obtain because it
0:03:22we use it uses because to survive
0:03:24so it does have its own
0:03:27so one or two controls and then we would use our utterance unique used
0:03:32to
0:03:34master the glucose uptake
0:03:37by our human one
0:03:40so from using those two controls plus of the trends unique used that i mean
0:03:45we would be able to compare the fluorescence intensity when all three cells
0:03:52uptake two and we did she and so we can assess the difference in c
0:03:59just to get a baseline model of the presence of the first effectively only answer
0:04:04a question
0:04:05would be to grow up my chance to unit keys and media containing
0:04:10different types of fatty acids
0:04:13and then you
0:04:15and performing the same as the again
0:04:18so that you can take two and beattie g so that we can see any
0:04:21difference between these trends to unique used cell
0:04:25and how they uptake to and beattie g
0:04:28and
0:04:29if we see any differences in for example we use only acid or any other
0:04:34kind of fatty acid then we would be a little bit closer
0:04:38understanding
0:04:40the mechanism of q one function